LOCUS       V003381                 4438 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V003381
VERSION     V003381
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Thermosensitive suicide vectors for gene replacement in
            Streptococcus suis
  JOURNAL   Plasmid 46 (2), 140-148 (2001)
   PUBMED   11591139
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Takamatsu D, Osaki M, Sekizaki T.
  TITLE     Direct Submission
  JOURNAL   Submitted (08-FEB-2001) Daisuke Takamatsu, National Institute of
            Animal Health, Laboratory of Molecular Bacteriology; Kannondai
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743,
            Fax:81-298-38-7743)
REFERENCE   3  (bases 1 to 4438)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4438)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plasmid";
            date: "2001"; volume: "46"; issue: "2"; pages: "140-148"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (08-FEB-2001) Daisuke Takamatsu, National Institute of Animal
            Health, Laboratory of Molecular Bacteriology"; volume: " Kannondai
            3-1-1, Tsukuba, Ibaraki 305-0856, Japan
            (E-mail:p1013dt@niah.affrc.go.jp, Tel:81-298-38-7743, Fax"; pages:
            "81-298-38-7743"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4438
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(133..144)
                     /label="Factor Xa site"
                     /note="Factor Xa recognition and cleavage site"
     CDS             528..689
                     /codon_start=1
                     /gene="orfC"
                     /product="OrfC"
                     /label="orfC"
                     /protein_id="BAB64888.1"
                     /translation="MVISESKKRVMISLTKEQDKKLTDMAKQKGFSKSAVAALAIEEYA
                     RKESEQKK"
     gene            528..689
                     /gene="orfC"
                     /label="orfC"
     CDS             756..1454
                     /codon_start=1
                     /gene="repAts"
                     /product="RepAts"
                     /label="repAts"
                     /protein_id="BAB64889.1"
                     /translation="MAIKNTKARNFGFLLYPDSIPNDWKEKLESLGVSMAVSPLHDMDE
                     KKDKDTWNNSNIIQNGKHYKKPHYHVIYIARNPVTIESVRNKIKRKLGNSSVAHVEILD
                     YIKGSYEYLTHESKDAIAKNKHIYDKKDILNINDFDIDRYITLDESQKRELKNLLLDIV
                     DDYNLVNTKDLMAFIRLRGAEFGILNTNDVKDIVSTNSSAFRLWFEGNYQCGYRASYAK
                     VLDAETGEIK"
     gene            756..1454
                     /gene="repAts"
                     /label="repAts"
     primer_bind     2036..2052
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    2053..2109
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(2122..2138)
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     protein_bind    complement(2146..2162)
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(2170..2200)
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(2215..2236)
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     rep_origin      complement(2524..3112)
                     /direction=LEFT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             complement(3575..4222)
                     /gene="cat"
                     /label="Chloramphenicol acetyltransferase"
                     /note="Chloramphenicol acetyltransferase from
                     Staphylococcus aureus. Accession#: P00485"
ORIGIN
        1 actagttatc ggcataatcg ttaaaacagg cgttatcgta gcgtaaaagc ccttgagcgt
       61 agcgtggctt tgcagcgaag atgttgtctg ttagattatg aaagccgatg actgaatgaa
      121 ataataagcg cagcgccctt ctatttcggt tggaggaggc tcaagggagt atgagggaat
      181 gaaattccct catgggtttg attttaaaaa ttgcttgcaa ttttgccgag cggtagcgct
      241 ggaaaatttt tgaaaaaaat ttggaatttg gaaaaaaatg gggggaaagg aagcgaattt
      301 tgcttccgta ctacgacccc ccattaagtg ccgagtgcca atttttgtgc caaaaacgct
      361 ctatcccaac tggctcaagg gtttaagggg tttttcaatc gccaacgaat cgccaacgtt
      421 ttcgccaacg ttttttataa atctatattt aagtagcttt attgttgttt ttatgattac
      481 aaagtgatac actaacttta taaaattatt tgattggagt tttttaaatg gtgatttcag
      541 aatcgaaaaa aagagttatg atttctctga caaaagagca agataaaaaa ttaacagata
      601 tggcgaaaca aaaaggtttt tcaaaatctg cggttgcggc gttagctata gaagaatatg
      661 caagaaagga atcagaacaa aaaaaataag cgaaagctcg cgtttttaga aggatacgag
      721 ttttcgctac ttgtttttga taaggtaatt atatcatggc tattaaaaat actaaagcta
      781 gaaattttgg atttttatta tatcctgact caattcctaa tgattggaaa gaaaaattag
      841 agagtttggg cgtatctatg gctgtcagtc ctttacacga tatggacgaa aaaaaagata
      901 aagatacatg gaataatagt aatattatac aaaatggaaa gcactataaa aaaccacact
      961 atcacgttat atatattgca cgaaatcctg taacaataga aagcgttagg aacaagatta
     1021 agcgaaaatt ggggaatagt tcagttgctc atgttgagat acttgattat atcaaaggtt
     1081 catatgaata tttgactcat gaatcaaagg acgctattgc taagaataaa catatatacg
     1141 acaaaaaaga tattttgaac attaatgatt ttgatattga ccgctatata acacttgatg
     1201 aaagccaaaa aagagaattg aagaatttac ttttagatat agtggatgac tataatttgg
     1261 taaatacaaa agatttaatg gcttttattc gccttagggg agcggagttt ggaattttaa
     1321 atacgaatga tgtaaaagat attgtttcaa caaactctag cgcctttaga ttatggtttg
     1381 agggcaatta tcagtgtgga tatagagcaa gttatgcaaa ggttcttgat gctgaaacgg
     1441 gggaaataaa atgacaaaca aagaaaaaga gttatttgct gaaaatgagg aattaaaaaa
     1501 agaaattaag gacttaaaag agcgtattga aagatacaga gaaatggaag ttgaattaag
     1561 tacaacaata gatttattga gaggagggat tattgaataa ataaaagccc cctgacgaaa
     1621 gtcgaagggg gtttttattt tggtttgatg ttgcgattaa tagatctcgg tgatgacggt
     1681 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1741 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     1801 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     1861 cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag gctgcgcaac
     1921 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     1981 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     2041 acgacggcca gtgaattcga gctcggtacc cggggatcct ctagagtcga cctgcaggca
     2101 tgcaagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     2161 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     2221 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     2281 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     2341 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     2401 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     2461 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     2521 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     2581 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     2641 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     2701 aagcgtggcg ctttctcaat gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     2761 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     2821 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     2881 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     2941 gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt
     3001 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     3061 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     3121 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     3181 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     3241 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     3301 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     3361 cgtgtagata actagtttcc gataattcga tgggttccga ggctcaacgt caataaagca
     3421 attggaataa agaagcgaaa aaggagaagt cggttcagaa aaagaaggat atggatctgg
     3481 agctgtaata taaaaacctt cttcaactaa cggggcaggt tagtgacatt agaaaaccga
     3541 ctgtaaaaag tacagtcggc attatctcat attataaaag ccagtcatta ggcctatctg
     3601 acaattcctg aatagagttc ataaacaatc ctgcatgata accatcacaa acagaatgat
     3661 gtacctgtaa agatagcggt aaatatattg aattaccttt attaatgaat tttcctgctg
     3721 taataatggg tagaaggtaa ttactattat tattgatatt taagttaaac ccagtaaatg
     3781 aagtccatgg aataatagaa agagaaaaag cattttcagg tataggtgtt ttgggaaaca
     3841 atttccccga accattatat ttctctacat cagaaaggta taaatcataa aactctttga
     3901 agtcattctt tacaggagtc caaataccag agaatgtttt agatacacca tcaaaaattg
     3961 tataaagtgg ctctaactta tcccaataac ctaactctcc gtcgctattg taaccagttc
     4021 taaaagctgt atttgagttt atcacccttg tcactaagaa aataaatgca gggtaaaatt
     4081 tatatccttc ttgttttatg tttcggtata aaacactaat atcaatttct gtggttatac
     4141 taaaagtcgt ttgttggttc aaataatgat taaatatctc ttttctcttc caattgtcta
     4201 aatcaatttt attaaagttc atttgatatg cctcctaaat ttttatctaa agtgaattta
     4261 ggaggcttac ttgtctgctt tcttcattag aatcaatcct tttttaaaag tcaatattac
     4321 tgtaacataa atatatattt taaaaatatc ccactttatc caattttcgt ttgttgaacc
     4381 attatatcac attatccatt aaaaatcaaa caaattttca tcaagctcta gttcggtg
//
